Coding

Part:BBa_K1321005:Experience

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-07)


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Applications of BBa_K1321005

The WPI 2016 iGEM Team (http://2016.igem.org/Team:WPI_Worcester) used this part for arsenic absorption experiments, and quantified the results.


The efficiency of the arsenic absorbing ability was measured by testing the concentration of the remaining arsenic in the solution. A sample image of the testing result is shown below:
T--WPI_Worcester--arsenic.jpg

The yellow color indicates the arsenic concentration, with a lower concentration producing a lighter color. A visual scale with corresponding concentration is shown below:
T--WPI_Worcester--arsenic_scale.png

The concentration decided by this method is usually estimated visually; however, we used ImageJ to obtain a numeric value for each shade of the yellow color (in the visual scale above), from which we were able to create two functions that approximate the relationship between color and concentration. For the experimental trials, the color was converted to numbers in the same manner.
T--WPI_Worcester--log_scale_.png T--WPI_Worcester--arsenic_log.png
A logarithmic approximation was generated using all data points obtained from the visual scale. The function was then used to solve for the arsenic concentration on each test strip (y = color, x = concentration). The average concentration is shown on the right (0.1 < standard error < 0.4).

Since all concentrations were below 100 mg/L, another linear approximation was generated using the first five data points only, shown below on the left. The concentrations calculated based on this function is shown below on the right (0.15 < standard error < 0.65).
T--WPI_Worcester--linear_scale_.png T--WPI_Worcester--arsenic_linear.png
The concentrations calculated using the logarithmic function is lower than using the linear function; however, the results are very consistent. Clone 1 and Clone 2 (with the chelator) show a lower arsenic concentration than the negative control in both results, indicating that they both were able to absorb a certain amount of arsenic in the solution.

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